Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Analysis of human proximal tubular albumin reuptake function. Albumin uptake by human proximal tubular epithelial cells after 15 min incubation at 37 °C with 50 µg mL-1 of FITC-albumin (green) added to the channel under static conditions ( A ) and fluid shear stress conditions ( B ). Mean Fluorescence Intensity (MFI) of RPTEC/TERT1 cells 24 h after static conditions versus fluid shear stress conditions showing a significant increase in FITC-Albumin fluorescence signal ( C ) (***p ≤ 0.001). Transcriptome profiling provides insight to differentially expressed genes corresponding to endocytosis process.

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Incubation, Shear, Fluorescence

Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Fluorescent transporter substrate Calcein-AM and CMFDA amasses in RPTEC/TERT1 cells under static conditions and dissipates with the application of FSS. Figure ( B ) and ( D ) sample fluorescence images demonstrate decreased in Calcein-AM and CMFDA accumulation in cells after 24 h of FSS treatment prior to staining compared to static channels ( A and C ) (Bar = 100 µm). ( E ) Quantitative analysis of Mean Fluorescence Intensity (MFI) of Calcein-AM showed a significant decrease after cells were treated with 24 h of FSS versus static environment (yellow filled bars). Indicating the increased efflux activity after being kept under fluidic conditions (Bar = 100 µm; n = 4, ***p ≤ 0.001).

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Fluorescence, Staining, Activity Assay

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Amplicon specificity for candidate reference genes determined by (a) melting curve analysis and (b) agarose gel electrophoresis. Gel images are cropped. Full‐length gel images are available in the Supporting information Material. ACTB, β‐actin; B2M, β‐2‐microglobulin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; HPRT, hypoxanthine‐guanine phosphoribosyltransferase; SDHA, succinate dehydrogenase complex, subunit A; HGF‐hTert, human telomerase reverse‐transcriptase immortalized human gingival fibroblasts; THP‐1, human acute leukemia‐derived monocytes; TIGK, telomerase immortalized gingival keratinocytes

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Amplification, Agarose Gel Electrophoresis, Reverse Transcription, Derivative Assay

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Mean quantification cycles (Cq) from tested reference genes across all experimental conditions ( n = 12). Boxplots show median, interquartile range (box), and minimum and maximum values (whiskers). HGF‐hTert, human telomerase reverse‐transcriptase immortalized human gingival fibroblasts; THP‐1, human acute leukemia‐derived monocytes; TIGK, telomerase immortalized gingival keratinocytes

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Reverse Transcription, Derivative Assay

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Overall results for the proposed normalization approach. (a) Telomerase immortalized gingival keratinocytes. (b) Macrophage‐like human acute leukemia‐derived monocyte (THP‐1) cells. (c) Human gingival fibroblasts. Graphs show rank sums of three different normalization algorithms Normfinder, Bestkeeper, and geNorm. The associated table presents keystone data for Bestkeeper calculations. BK, Bestkeeper; Cq, mean quantification cycles; CV, coefficient of variation; GM, geometric mean; r , coefficient of correlation; r 2 , coefficient of determination; SD, standard deviation (Pfaffl et al., )

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Derivative Assay, Standard Deviation

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Ranking orders for expression stability by means of different normalization algorithms in human telomerase reverse‐transcriptase immortalized human gingival fibroblasts (HGF‐hTert) cells. Normfinder (a) and Bestkeeper (c) calculate standard deviations (SDs) across experimental conditions. GeNorm (b) calculates the M value from pairwise mean quantification cycle (Cq) variation

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Expressing, Reverse Transcription

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Ranking orders for expression stability by means of different normalization algorithms in telomerase immortalized gingival keratinocyte (TIGK) cells. Normfinder (a) and Bestkeeper (c) calculate standard deviations (SD) across experimental conditions. GeNorm (b) calculates the M value from pairwise mean quantification cycle (Cq) variation

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Expressing

Journal: Clinical and Experimental Dental Research

Article Title: Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research

doi: 10.1002/cre2.525

Figure Lengend Snippet: Expression fold changes (2 (−ΔΔCq) ) of ACTB between three experimental conditions and the low glucose (LG) group. Calculations were performed with a common housekeeping gene and a combination of the most stable reference genes. Cq, mean quantification cycle; HG, high glucose; LPS, lipopolysaccharide; TIGK, telomerase immortalized gingival keratinocyte

Article Snippet: Telomerase immortalized gingival keratinocyte cell line (TIGK‐hTert; ATCC CRL‐3397, Manassas, VA, USA) was cultured in Dermal Cell Basal Medium (ATCC) and supplemented with Keratinocyte Growth Kit (ATCC PCS‐200‐040).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Molecular Networking for Drug Toxicities Studies: The Case of Hydroxychloroquine in COVID-19 Patients

doi: 10.3390/ijms23010082

Figure Lengend Snippet: Cytotoxicity and visualization of in vitro HCQ metabolism using molecular networking. Differentiated HepaRG and RPTEC were incubated with HCQ (10 µM) for 48h (at least three experiments in both cell lines). ( A ) Cytotoxicity was evaluated using XTT and NRU assays. Cell viability was calculated compared to control conditions after 48 h of treatment. ( B ) The molecular network. Each cell type is depicted in a specific color: HepaRG cells in grey and RPTEC in pink. ( C ) Details of the specific HCQ-containing cluster. Nodes are labelled with the exact protonated mass ( m/z ) and the links are labelled with the exact mass shift. Proposed metabolites of HCQ structure are linked to the corresponding nodes.

Article Snippet: The cells were cultured in DMEM/F12 supplemented with hTERT Immortalized RPTEC Growth Kit (LGC Standards, Molsheim, France).

Techniques: In Vitro, Incubation, Control

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Expression of CD4, CD8, CD20, CD56, CD68, CD117, and CD177 by immune cells infiltrated tumor tissues of GC patients. Representative images of immune markers staining in immune cells from GC samples are shown at ×200 (100 mm) original magnification. Positive expression of CD4 (a), CD8 (b), CD20 (c), CD56 (d), CD68 (e), CD117 (f), and CD177 (g) infiltrated in tumor tissues with a regular distribution. In order to show the distribution from immune cells more clearly, we used computerized imaging system Image-Pro Plus version 6.2 to highlight positive areas in different colors.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Expressing, Staining, Imaging

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: The schematic diagram of the distribution of immune cells. The blue points represent CD20 + B cells, the yellow points represent CD4 + T cells, the orange points represent CD117 + mast cells, the green points represent CD68 + macrophages, the pink points represent CD8 + T cells, the black points represent CD56 + NK cells, and the red points represent CD177 + neutrophils. Although not all immune cells are distributed according to this fixed law, this pattern can basically reflect the general characteristics of their distribution.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques:

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Differences in immune marker positive area/total area between the two groups (survival time of patients in group A was less than 1 year and group B survival time was more than 5 years) by the rank sum test. (a) CD4 + T cells ( P < 0.001). (b) CD8 + T cells ( P < 0.001). (c) CD20 + B cells ( P < 0.001). (d) CD68 + macrophages ( P < 0.001). (e) CD117 + mast cells ( P < 0.001). (f) CD177 + neutrophils ( P < 0.001).

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Marker

Journal: American Journal of Cancer Research

Article Title: Antineoplastic efficacy profiles of avapritinib and nintedanib in KIT D816V + systemic mastocytosis: a preclinical study

doi:

Figure Lengend Snippet: Specification of monoclonal antibodies (mAb)

Article Snippet: SCF-R/KIT , CD117 , 104D2 , mouse , IgG1 , PE , BD Biosciences.

Techniques: